PyFli

Unified platform for Fluorescence Lifetime Imaging (FLI) data processing, simulation, and visualization

PyFli is an open-source Python package I authored and published on PyPI for the unified, scalable processing and analysis of Fluorescence Lifetime Imaging (FLI) data.

It supports multiple acquisition methods — ICCD, SPAD, and TCSPC — and provides a range of analytical approaches, from traditional non-linear least squares fitting to deep-learning-based inference methods, all under a single, consistent interface.

A new release is scheduled for August 31, 2026. Check pyfli.org for the latest documentation, or install the current release from PyPI:

pip install pyfli-lib